Table 5.

Complementation experiment for LamB and PBP3.

Transformed plasmid	Strain	MIC value of CAZ-AVI (mg/L)				MIC value of CAZ (mg/L)
		Mutant strain	Mutant stain + empty vector	Mutant stain complemented with wild-type gene	MIC Fold change	Mutant strain	Mutant stain + empty vector	Mutant stain complemented with wild-type gene	MIC Fold change
pAClamB	P77-IR	16	16	16	1	1024	1024	1024	1
	C4-IS	16	16	8	0.5	512	512	256	0.5
	BL94-IR	16	16	8	0.5	512	512	256	0.5
	Q38-IR	64	64	32	0.5	512	512	256	0.5
pAC ftsI	84082-IR	128	128	4	0.03	2048	2048	1024	0.5

Note: The CAZ-AVI resistant strains after induction were named following the format of ‘original strain name -IR’ (e.g. P77-IR). The induced strains that were still susceptible to CZA-AVI are named as ‘original strain name -IS’ (e.g. P152-IS).