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. 2021 Sep 28;25(5):296–311. doi: 10.1080/19768354.2021.1978543

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Chondrogenic differentiation capabilities of the nine groups of MSCs. (A)–(D) MSCs in a 21-day micromass culture with non-differentiation control (−) and chondrogenic differentiation media (+). (A) and (C) Micromasses stained with Alcian blue. (B) and (D) Immunodetection of type II collagen assessed in micromasses. Scale bar: 100 μm. (E) Quantitative real-time polymerase chain reaction measurements of the expression levels of the chondrogenic markers Sox9 and Col2a1. mRNA levels were measured in three independent samples (n = 3). Data are expressed as mean ± standard deviation. *P < .05. FA, fibrous ankylosis; BA, bony ankylosis; BM, bone marrow; MSCs, mesenchymal stromal cells.