Table 3.

Represents the number and type of unique variations/substitutions in the nucleotide and amino acid of all the genes with respect to their position. Additionally, Synonymous mutation (SN) and Non-Synonymous mutation (NS) that were observed within the Indian BEFV isolate in comparison to globally available sequences are also summarized.

Gene	Gene product	Unique nucleotide variation	Unique amino acid substitution	Substitution type with no.		Substituted amino acid position and change
				SN	NS	SN		NS
						Position	Change	Position	Change
N	Nucleoprotein	7	0	0	0	–	–	–	–
P	Phosphoprotein	14	9	5	4	59	P/T→ A	45	E/R → G
						154	V→I	115	S/L/F→
						178	I/V/P→L	152	P
						183	N/R→K	173	S→L
						272	I→V		K→N
M	Matrix protein	0	0	0	0	–	–	–	–
G	Glycoprotein	3	1	1	0	18	K → R	–	–
GNS	Non-structural Glycoprotein	25	8	5	3	16	T → N	490	E → G
						37	L→ M	514	E → K
						177	N→S	520	K → I
						380	I → V
						504	L→V
α1	Alpha 1 protein	4	2	2	–	51	A→ V	–	–
						83	K→ R
α 2	Alpha 2 protein	4	1	1	0	40	E→ D	–	–
β	Beta protein	1	0	0	0	–	–	–	–
γ	Gamma protein	0	0	0	0	–	–	–	–
L	Large subunit of polymerase protein	18	10	6	4	12	H→N	1635	K→E
						1099	K→R	1937	K→N
						1564	E→D	2072	G/E→R
						1766	D→E
						2044	L→ I
						2058	K→R
						2107	K→Q

^*SN depicts synonymous mutation and NS shows non-synonymous mutation. All amino acid is denoted by its standard symbol used universally.