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. 2021 Oct 11;25(5):312–322. doi: 10.1080/19768354.2021.1987320

Figure 1.

Figure 1

. UCH-L1 and UCH-L3 regulate the pluripotency markers in RWPE1 cells. (A) The expression of pluripotency markers, including Oct4, Nanog, Bmi1, Klf4, and Sox2, were examined by immunoblotting in RWPE1 stable cell lines as indicated. β-actin was used as the loading control. (B) Real-time quantitative RT-PCR analysis of Oct4, Nanog, Bmi1, Klf4, and Sox2 in RWPE1 stable cell lines. The values shown are the mean ± SD of three independent experiments, and the p-value was obtained from a Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001.