Fig. 2.

Dendritic cells (DCs) from mixed allogeneic cultures display normal, immature morphology after prolonged cultivation time. DCs were differentiated from monocytes of four unrelated healthy donors. Fully differentiated iDCs were either cultured in single or mixed donor cultures for 72 hours. Cultures of mixed allogeneic DCs displayed no particular features in comparison to single donor ones. Normal immature morphology with the absence of cell clustering or adherence, which is characteristic of the DC maturation/activation process, was observed in both cases