Fig. 3.

Dendritic cells (DCs) from mixed allogeneic cultures preserve their immature phenotype and respond readily to maturation stimulus. DCs were prepared and cultured as described in Material and methods. Differentiated from monocytes, isolated from four unrelated healthy donors, the resulting iDCs were cultured both individually and in a co-culture, for 48 hours, either in the absence of presence of poly I:C (20 μg/ml). Subsequently, cells were harvested and stained for the depicted surface markers. Data show phenotypic characterizations of 3 out of 4 DCs from individual donors (only due to the clarity of the overview) and of DCs from a mixed culture. Numbers represent the mean fluorescence intensities (MFIs) for each depicted surface marker