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. 2021 Oct 21;15:743484. doi: 10.3389/fnbeh.2021.743484

FIGURE 4.

FIGURE 4

Effect of ZI silencing on spatial cognitive ability. (A,D) The basic preference of mice for two identical objects were examined before training. The mice in all groups did not exhibit an innate preference for the two identical objects. The two-tailed paired t-test was used to compare the preference score of mice for object-1 and object-2. (B,E) The investigating time of mice for two identical objects were examined after training. The non-stressed mice spent more investigating time on the novel location object than that on the familiar location object, whereas the stressed mice spent more investigating time on the novel location object only in AAV-GFP group, and showed the opposite phenotype in AAV-Tet-tox group. Two-way ANOVA with bonferroni post hoc test was used to compare the investigating time for the novel and familiar location object. (C,F) The RI of two identical objects by mice was recorded after training. The stressed mice showed similar RI to the non-stressed mice in AAV-GFP group, while the RI was significantly decreased in stressed mice compared to the non-stressed mice in AAV-Tet-tox group. The two-tailed unpaired t-test was used to compare the RI between non-stressed and stressed mice. **p < 0.01, ***p < 0.001.