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. 2021 Nov 4;19(11):e3001423. doi: 10.1371/journal.pbio.3001423

Fig 5. Association of mutant pORF19 proteins with KSHV capsids produced in insect cells.

Fig 5

(A) Baculovirus-produced KSHV capsids lacking the portal protein pORF43 and containing either wt or mutant pORF19 were purified by gradient ultracentrifugation from cell lysates as described before [44] and analyzed by western blot. The quantity of capsids loaded was normalized to the MCP pORF25 carrying a V5 tag (153.4 kD) and the triplex protein pORF26 (30 kD). Immunoblot analysis of GFP-tagged pORF19 of a representative experiment is shown (88.2 kD). (B) Capsid productions were performed in 3 independent biological replicates, intensities of pORF19 bands were quantified, expressed in percent of wt pORF19, and mean values with error bars representing SD shown. The underlying data can be found in S2 Data. KSHV, Kaposi’s sarcoma-associated herpesvirus; MCP, major capsid protein; SD, standard deviation; wt, wild-type.