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. 2021 Nov 4;16(11):e0258204. doi: 10.1371/journal.pone.0258204

Fig 3. Inhibiting IDO-1 with 1-MT or INCB24360 reduced the proportion of M1-like but not M2-like RAW264.7 cells.

Fig 3

(A) The typical morphology of RAW264.7 cells treated with IFN-γ, 1-MT and INCB24360 for 24 h. More round and small RAW264.7 cells were observed in the 1-MT and INCB24360 groups compared to the IFN-γ and control groups. The percentage of the total polarized, M1-type-cells (ramified), and M2-type-cells (slender) in the control, IFN-γ, 1-MT and INCB24360 groups. N ≥ 5. Scale bars, 100 μm. (B) The phalloidin Alexa-488 staining of RAW264.7 cells treated with IFN-γ, 1-MT or INCB24360 for 24 h. The cellular perimeters in the control, IFN-γ, 1-MT and INCB24360 groups. The density of the filopodia on the membrane of RAW264.7 cells in the control, IFN-γ, 1-MT and INCB24360 groups. n ≥30. Scale bars, 30 μm. One-way ANOVA; all data are expressed as the means ± SEM. *, P<0.05, **, P<0.01; ns, no significant difference.