Fig 5. ZAP cofactors KHNYN and TRIM25 bind most efficiently to wild type ZAP-L.

Upper left panel: Representative western blot of GFP-ZAP isoforms and mutant proteins expressed in HEK293T ZAP KO cells. Upper right panel: GFP-ZAP, TRIM25 and KHNYN co-immunoprecipitated using GFP-binding magnetic beads. Input and pulldown samples were stained for GFP as well as endogenous KHNYN and TRIM25. Lower panels: quantification of KHNYN and TRIM25 co-immunoprecipitated with GFP-ZAP normalized to the relative GFP signal. Mean of n = 5 + SD. * p < 0.05 for the sample compared to ZAP-L. * p < 0.05 for the comparisons demarked by the lines.