FIG. 2.

Amplification of hypervariable regions within the HSV-1 genome. (A) PCR amplification of regions containing ReI, ReIII, ReIV, and ReVII was performed with DNA from various HSV-1 (strain F) subclones. Amplicons were electrophoresed on a 2% agarose gel and were visualized by ethidium bromide staining. Ten representative samples from 24 subclones analyzed are shown. Lane F, parental strain HSV-1 F; lanes 1 to 10, HSV-1 F subclones; lane C, water control; lanes M, 100-bp molecular size marker. Numbers on the left are in base pairs. (B) Autoradiogram of DNA in gel from panel A after Southern blot transfer and hybridization with a Re-specific probe.