TABLE 1.

Primers used for amplification and detection of HSV-1 reiterations

Genome region	Primera	Optimal annealing temp (°C)	Sequence (5′→3′)	Position in genomeb
“a” sequence	ReIF	72	GCCGCCACCGCTTTAAAGGGCCGC	125976–125999 and 152234–152257
	ReIR		GTGCTCTGTTGGTTTCACCTGTGGCAGC	126368–126395 and 151838–151865
“a” sequence	ReIIIF	72	TCTCTACCTCAGTGCCGCCAATCTCAGGTC	126742–126771 and 151462–151491
	ReIIIR		CGAAGACGCAATAAACGGCAACAACCTG	127171–127198 and 151035–151062
US1	ReIVUS1F	64	TCCGACGACAGAAACCCACC	132333–132352
	ReIVUS1R		GTCCCGGAGGACCACAGTGG	132615–132634
US12	ReIVUS12F	58	TTTTTGCACGGGTAAGCAC	145853–145871
	ReIVUS12R		TGGTGTCCAGGAAGGTGTCC	145535–145554
US10-US11	ReVIIUS1011F	56	AGCGTATGCTCCATGTTGTG	144697–144716
	ReVIIUS1011R		CGAGAACCTAGGGAACCCA	144928–144946
ReI	ReI probe	37	CCGCTCCTCCCC
ReIII	ReIII probe	37	CCGCCCCTCGCCCCCTC
ReIV	ReIV probe	37	CCCCCTCCTCCACCCCCGCGTC
ReVII	ReVII probe	37	CCCCGGTCTCCCCGGGAG

^aF, forward; R, reverse. 

^bPositions correspond to the genomic HSV-1 sequence HE1CG (accession no. X14112).