Table 4.

The mean Ct value of SARS-CoV-2 RdRP and E gene (mean ± standard deviation) using one-step versus two-step RT-qPCR.

Sample dilutions	Ct, RdRP gene		Ct, E gene
	one-step	two-step	one-step	two-step
10−1	26.22 ± 0.28	29.44 ± 0.11	23.38 ± 0.11	28.88 ± 0.04
10−2	29.88 ± 0.01	32.71 ± 0.17	27.28 ± 0.51	33.54 ± 0.42
10−3	32.88 ± 0.29	35.61 ± 0.16	30.71 ± 0.21	ND
10−4	36.32 ± 0.26	ND	33.35 ± 0.66	ND

ND: not detectable.