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. Author manuscript; available in PMC: 2021 Nov 4.
Published in final edited form as: Cell Rep. 2021 Apr 13;35(2):108986. doi: 10.1016/j.celrep.2021.108986

Figure 6. The VP40-nucleic acid complexes have well-defined stoichiometry.

Figure 6.

(A) The deconvoluted mass spectrum of the VP40 monomer/dimer SEC fraction as measured by size-exclusion chromatography-nMS (SEC-nMS).

(B) The mass spectrum of the VP40 endogenous ring SEC fraction as measured by nMS, yielding a broad m/z signal that could not be resolved. The m/z range highlighted in blue was mass selected for dissociation in (C).

(C) The deconvoluted mass spectrum of the heterogeneous ring signal highlighted in (B) after mass-selection and fragmentation by surface-induced dissociation (SID).

(D-G) The deconvoluted mass spectra of the VP40 monomer/dimer after incubation with 3′ UTR-inspired DNA oligos (Table S2), as measured by SEC-nMS. For each detected complex, cartoons indicate the VP40 oligomeric state and the number of bound DNA oligos. In (F), each red X denotes a putative tetramolecular G-quadruplex of AGGGG oligos.