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Percentage of methylation using Illumina (850K Epic Array) or targeted pyrosequencing of the 3 CpGs selected for validation in the discovery and validation cohorts in (A) women and (B) men. Association of the 3 CpGs methylation with the risk of having chronic low back pain using a logistic regression (C). Unpaired two-tailed (discovery cohort) or one-tailed (validation cohort) t test, * = P < 0.05, ** = P < 0.01, *** = P < 0.001.
