Table 1. Meiotic competence of in vitro-grown bovine oocytes in cocultured denuded oocytes and mural granulosa cells (DO+MGCs) and oocyte-cumulus cell-mural granulosa cell complexes (OCGCs) after in vitro maturation.

In vitro growth (day)	Types of complexes 1)	Number of oocytes used	Number (%) of oocytes at each stage 4)
			FC	SC	GV	ED	LD	MI	AI-TI	MII	DG
0 2)	-	30	3 (10)	23 (77)	4 (13)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
14	DO+MGCs	18	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	2 (11)	1 (6)	13 (72)	2 (11)
	OCGCs	23	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	2 (9)	0 (0)	19 (83)	2 (9)
In vivo3)	-	27	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	4 (15)	0 (0)	23 (85)	0 (0)

¹⁾ DO+MGCs and OCGCs collected from early antral follicles (0.5–0.7 mm) were subjected to in vitro maturation culture after in vitro growth culture. ²⁾ Oocytes were collected from early antral follicles for culturing. ³⁾ Fully grown oocytes were collected from antral follicles (4–6 mm). ⁴⁾ FC, filamentous chromatin; SC, stringy chromatin; GV, germinal vesicle I–IV; ED, early diakinesis; LD, late diakinesis; MI, metaphase I; AI-TI, anaphase I and telophase I; MII, metaphase II; DG, degeneration.