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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Adv Healthc Mater. 2021 Sep 5;10(21):e2101048. doi: 10.1002/adhm.202101048

Figure 3. Spheroid-produced GFs are retained within sulfated alginate hydrogels.

Figure 3.

(a) MSC spheroids were entrapped in sulfated alginate hydrogels. (b) No differences were observed in cell viability by LIVE/DEAD stain of MSC spheroids entrapped in sulfated and non-sulfated alginate on day 8 (scale bar = 500μm). Live cells are green, dead cells are red. (c) Ratio of live cell fluorescence to total cell fluorescence revealed no differences. (d) Metabolic activity is comparable for MSC spheroids entrapped in sulfated and non-sulfated alginate on day 8. (e) MSC-secreted VEGF is retained in sulfated alginate hydrogels on days 1 and 4. (f-g) Retained MSC spheroid cytokines in sulfated and non-sulfated hydrogels. Data are mean ± SD (n=4–5). Groups with statistically significant differences do not share the same letters; ns denotes no significance among all groups.