(a) MSC spheroid conditioned media was entrapped in sulfated and non-sulfated hydrogels, and IC-21 macrophages were then seeded on the hydrogels. (b) Total macrophages recovered from the hydrogel. (c) M1 polarized macrophages (CD86+, iNOS+) and (d) M2 polarized macrophages (CD86-, CD206+, ARG1+) were quantified via flow cytometry. Data are mean ± SD (n=6), ns denotes no significance among all groups.