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. 2021 Nov 4;11:21690. doi: 10.1038/s41598-021-00699-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Colonization of B. subtilis yb-114,246. (a) B. subtilis yb-114,246 in the distal ileum was detected with FISH and (b) the total number of B. subtilis yb-114,246 estimated based on a standard curve by qPCR assay (c). Values represented by vertical are means, with standard errors are represented by vertical bars. Significant differences from the control group were determined by one-way ANOVA followed by Duncan’s multiple comparison tests: superscript capital letters in columns mean P < 0.01. The Microsoft Office Excel 2010 was used to as the stamp software.