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. 2021 Oct 6;53(10):1547–1558. doi: 10.1038/s12276-021-00674-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a circSPG21-target microRNA was predicted based on the intersection of the TargetScan, miRanda, and RNAhybrid software data. b A ceRNA network diagram was drawn using Cytoscape. c Expression of microRNAs after circSPG21 knockdown, as detected by real-time quantitative polymerase chain reaction (RT-qPCR), **p < 0.01. Data are presented as the mean ± S.D., and the p values were determined by a two-tailed unpaired Student’s t-test. d miR-1197 expression increased in degenerated tissues, as measured by RT-qPCR; n = 6, ***p < 0.001. Data are presented as the mean ± S.D., and the p values were determined by a two-tailed unpaired Student’s t-test. e Left, the expression of miR-1197, as measured by fluorescence in situ hybridization (FISH), in degenerated tissues was higher than that in control tissues (scale bar, 100 μm). Right panel, the fluorescence intensity of miR-1197 was stronger in degenerated tissue (two different donors), **p < 0.01. Data are presented as the mean ± S.D., and the p values were determined by a two-tailed unpaired Student’s t-test. The miR-1197 probe was labeled with Alexa Fluor 488. f circSPG21 and miR-1197 were localized in the cell, and both were seen in the cytoplasm (scale bar, 25 μm). g The sites where circSPG21 and miR-1197 bind to each other were identified using CircInteractome. h The target genes of miR-1197 were predicted based on the intersection of TargetScan, miRDB, and miRTarBase results. i Expression of target genes after miR-1197 knockdown, as detected by RT-qPCR; *p < 0.05, **p < 0.01. Data are presented as the mean ± S.D., and the p values were determined by a two-tailed unpaired Student’s t-test. j The expression of ATP1B3, BZW1, and HNRNPR was detected in degenerated tissues using RT-qPCR; *p < 0.05, ***p < 0.001. Data are presented as the mean ± S.D., and the p values were determined by a two-tailed unpaired Student’s t-test. k The binding site of miR-1197 and ATP1B3 predicted by TargetScan.