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. 2021 Oct 25;53(10):1612–1622. doi: 10.1038/s12276-021-00690-z

Fig. 2. Low-dose DOX impairs Vegfr-2 translation and angiogenesis via REDD1 induction.

Fig. 2

VEGFR-1/2 expression and VEGF-A-induced angiogenesis were examined in HUVECs treated with DOX following transfection with control (siC) or Redd1 siRNA (siR). a, b Western blots of REDD1 and VEGFR-1/2 (a) as well as phosphorylated mTOR, 4E-BP1, and S6K (b). c Polysome profiling using sucrose density gradient ultracentrifugation. d qRT-PCR of Vegfr-1/2 mRNAs associated with high-molecular-weight polysomes (n = 4). e Western blots of phosphorylated VEGFR-2, ERK, and Akt in the HUVECs stimulated with VEGF-A. f Representative images of the migration (upper) and tube formation (lower) of the HUVECs stimulated with VEGF-A were obtained using Boyden chamber and Matrigel-based morphogenesis assays, respectively. g, h Quantitation of migration (g) and tube formation (h) was performed using ImageJ software (n = 4). Data are presented as the mean ± SD. **P < 0.01, ***P < 0.001; NS, not significant.