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Vc1c-mediated MCF-7 cell labelling. (A) Schematic of MCF-7 cell surface amine labelling with the peptide fluorescein-GRANGI using Vc1c. (B) Flow cytometry analysis of MCF-7 labelling with the peptide fluorescein-GRANGI. Untreated cells with neither peptide nor ligase added and cells with only peptide added were used as negative controls. The reaction was conducted at 37°C for 1 h using 50 μM fluorescein-peptide and 200 nM Vc1c.
