TABLE 1.

Kinetic parameters of VcAEP and Vc1a-1c.

	Enzyme	kcat (s−1)	Km (μM)	kcat/Km (s−1 M−1)	Substrate sequence	Enzyme concentration (μM)
Cyclization	VcAEP	0.01863	4.173	4,464	GISTKSIPPIE(EDANS)YRN-SLK(DABCYL)	0.438
	Vc1a	0.2025	13.77	14,700	GISTKSIPPIE(EDANS)YRN-SLK(DABCYL)	1.39
	Vc1b	0.03471	1.719	200,192	GISKPE(EDANS)SYAN-GIK(DABCYL)	0.06
	Vc1c	2.31	2.517	917,759	GISKPE(EDANS)SYAN-GIK(DABCYL)	0.044
Ligation	Vc1c	0.3761	33.68	11,166	N-terminal: RE(EDANS)AN-GI	0.044
					C-terminal: GVK(DABCYL)