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. 2021 Nov 4;11:21705. doi: 10.1038/s41598-021-00608-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Biological network of the MGI RNA-seq dataset following Gene Ontology (biological process) gene set enrichment analysis in GSEA (v4.0.3) and visualisation in Cytoscape (3.8.0). Each circle (node) represents a gene set and two nodes are connected by lines (edges) indicating shared genes. The size of a node and width of an edge are proportional to the number of genes enriched in a gene set and the number of genes shared between gene sets, respectively. Gene sets that are similar were annotated and clustered to form a biological theme using the AutoAnnotate App in Cytoscape. The most significantly enriched gene set is used to label a gene set cluster, defined by NES. Red node: gene set enriched in EPO4. Purple node label: top gene sets with NES > 1.90. The enrichment map was created with pathway FDR < 0.1, nominal P < 0.05 and Jaccard Overlap coefficient > 0.375 with combined constant k = 0.5.