Table 2.
Yield of essential oil yield obtained after 120 min of hydrodistillation (hydromodule 1:10 m/v) and EC50 values of essential oil from the aerial part (herb) of thyme, marjoram and oregano.
| Plant species | Shading | Essential oil yield, mL/100 g p.m. | EC50, mg mL−1/20 min incubation |
|---|---|---|---|
| Thyme | Unshaded | 2.32b ± 0.03 | 0.944a ± 0.001 |
| Shaded | 2.57a ± 0,09 | 0.852a ± 0.005 | |
| Marjoram | Unshaded | 1.51d ± 0.03 | 54.012e ± 1.051 |
| Shaded | 1.68c ± 0.03 | 19.972d ± 0.199 | |
| Oregano | Unshaded | 0.27e ± 0.01 | 7.449c ± 0.018 |
| Shaded | 0.32e ± 0.01 | 7.023b ± 0.054 | |
| ANOVA (p values) | |||
| Plant species | 0.00000 | 0.00000 | |
| Shading | 0.00000 | 0.00000 | |
| Plan species · shading | 0.00518 | 0.00000 | |
Values followed by the same letter do not significantly differ between the treatments, at P = 0.05 according to Duncan’s multiple range test.
c
EC50, mg∙mL−1 = concentration of extract necessary to neutralize 50% of initial concentration of DPPH radicals.