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. 2021 Oct 27;54(5):167–175. doi: 10.1267/ahc.21-00057

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2021 The Japan Society of Histochemistry and Cytochemistry

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Fig. 4. — Basement membrane (BM) structure. The BM structures of the soleus muscle were observed in the Aged Ex (−) (A and B) and the Aged Ex (+) (C and D) using immunohistochemistry with collagen IV (×20). Bar = 25 μm. B and D are extended images of black rectangles in A and C. Enlargement of the area of collagen IV-IR is observed in Aged Ex (+) rats (D) compared to Aged Ex (−) (B). The area (E) and intensity (F) of collagen IV-IR was measured in the Aged Ex (−) (n = 5) and the Aged Ex (+) (n = 4) using the stained images. *p < 0.05 vs. Aged Ex (−). Data are shown as the mean ± standard error of the mean (SEM).