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. 2021 Oct 27;54(5):167–175. doi: 10.1267/ahc.21-00057

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2021 The Japan Society of Histochemistry and Cytochemistry

This is an open access article distributed under the Creative Commons License (CC-BY-NC), which permits use, distribution and reproduction of the articles in any medium provided that the original work is properly cited and is not used for commercial purposes.

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Fig. 5. — Electron microscopy analysis. Longitudinal ultrathin sections of the soleus muscles were examined using transmission electron microscopy (TEM) in Aged Ex (−) (n = 5) and Aged Ex (+) (n = 4) rats. Muscle fiber (MS) and extracellular matrix (ECM) images of Aged Ex (−) (A and B) and Aged Ex (+) (C and D) rats are presented. B and D are extended images of the black rectangles in A and C (×7000). Bar = 200 nm (A–D). An increase in the width of the lamina densa was observed in Aged Ex (+) rats (D) compared to that in Aged Ex (−) (B). The width of the lamina densa (E) was measured in the Aged Ex (−) (n = 5) and the Aged Ex (+) (n = 4) rats. MS images of Aged Ex (−) (F) and Aged Ex (+) (G) rats are presented (×300). The width of the MS (H) was measured in the Aged Ex (−) (n = 5) and the Aged Ex (+) (n = 4) rats. Sarcomere images of Aged Ex (−) (I) and Aged Ex (+) (J) rats are presented (×2000). The width of the sarcomere (K) was measured in the Aged Ex (−) (n = 5) and the Aged Ex (+) (n = 4) rats. Bar = 5 μm (F, G, I and J). **p < 0.01 vs. Aged Ex (−). Data are shown as mean ± standard error of the mean (SEM).