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. 2021 Oct 22;9:766087. doi: 10.3389/fbioe.2021.766087

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Copyright © 2021 Li, Bai, Lai, Tian, Li, Sun and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Real-time quantitative PCR was performed to compare the expression of collagen-I, collagen-II and aggrecan genes in AFSCs cultured on aligned and random scaffolds. After 7 days of culture, the expression level of collagen-I in AFSCs cultured on aligned scaffolds was almost twice that of cells grown on random scaffolds. (B) The expression of collagen-II was similar in both AFSCs cultured on aligned and random scaffolds. (C) The expression pattern of aggrecan was similar to that of collagen-I; cells cultured on aligned scaffolds had approximately 0.4 times higher aggrecan gene expression than those grown on random scaffolds. (D) There was no apparent difference in cell proliferation between AFSCs cultured on aligned scaffolds and those on random scaffolds. ((A–D) Adapted from Liu et al. (2015)).