Fig. 1. Establishment of an organoid biobank and its applications.
a Applications for next-generation organoid biobanks in translational research. b Basic workflow for the generation of an organoid biobank. Key areas requiring careful consideration and detailed documentation include (1) donor information, (2) tissue of origin, (3) culture methodology and passage number, (4) generation of frozen stocks, (5) documentation of organoid growth and morphology, (6) molecular profiling of organoids and/or primary tissue, and (7) relevant clinical information. All images were created with BioRender.com.