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. 2021 Nov 4;12:6399. doi: 10.1038/s41467-021-26655-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a CCK-8 cytotoxicity analysis of HeLa cells treated with different formulations. b Western blotting analysis of glutathione peroxidase (GPX4) and GADPH. The samples derived from the same experiment and that blots were processed in parallel. c The analysis of caspase-3 activity in HeLa cells using Alexa 647-conjugated caspase-3 antibody. Pink: caspase-3; Blue: nuclei. d Live/dead cytotoxicity analysis of HeLa cells treated with different formulations. Green: live cells; Red: dead cells. e CCK-8 cytotoxicity analysis of 4T1 and HepG2 cells treated with different formulations. Data in (a), (b), and (e) were represented as mean values ± SD, n = 3 biologically independent samples in (a) and (b), n = 4 biologically independent samples in (e). A representative image of three biologically independent samples from each group is shown in (b–d). P values were calculated by using one-way ANOVA in (a), (b), and (e).