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. 2021 Oct 26;53(10):1623–1635. doi: 10.1038/s12276-021-00687-8

Fig. 4. Overexpression of SIRT6 ameliorated the migration, EMT phenotype, glycolysis activity, and metastasis of PDAC.

Fig. 4

a Cumulated data of the Transwell migratory assay of Panc-1pLKO and Panc-1shKLF10 cells with and without SIRT6 overexpression. Data are presented as the mean ± SE (** signifies p < 0.01). The experiments were repeated three times. b Cumulated data of directional migration over time (or. dist.) in Panc-1pLKO and Panc-1shKLF10 cells with and without SIRT6 overexpression. Data are presented as the mean ± SE (** signifies p < 0.01). The cell trajectory experiments were repeated three times. c Representative IVIS images of mice at 1–4 weeks after injection with ASPC-1pLKO and ASPC-1shKLF10 cells with and without SIRT6 overexpression as indicated. d Cumulated IVIS signal of at least six mice in each experimental group injected with ASPC-1pLKO and ASPC-1shKLF10 with and without SIRT6 overexpression. Data are presented as the mean ± SE (* represents p < 0.05). e Representative immunoblots of E-cadherin and mesenchymal and glycolytic protein expression in Panc-1pLKO and Panc-1shKLF10 cells with and without SIRT6 overexpression. β-Actin was used as the internal control. Quantitative analysis of at least three experiments is shown below the immunoblots. f Cumulated lactate production assay data of Panc-1pLKO and Panc-1shKLF10 cells with and without SIRT6 overexpression. Data are presented as the mean ± SE (** and *** signify p < 0.01 and p < 0.005, respectively). The experiments were repeated three times.