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. 2021 Nov 4;11:21328. doi: 10.1038/s41598-021-00724-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fibril formation of the CAHS1 protein in vitro. (A) ¹H-¹⁵N HSQC spectra of the CAHS1 protein at protein concentrations of 0.02, 0.1, 0.3, and 0.6 mM. (B) Typical HS-AFM images of CAHS1 protein fibrils. Left: the HS-AFM image of the mica surface with CAHS1 protein at a final concentration of 0.4 μM, about 3 min after injection into the observation solution. Right: the HS-AFM image about 2 min after the addition of CAHS1 protein at a final concentration of 3.3 μM.