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. 2021 Oct 22;11:769542. doi: 10.3389/fcimb.2021.769542

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Copyright © 2021 Bhattacharya and Nuttall

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neighbour-joining tree of class B evasin and evasin-like proteins. Accession numbers of proteins are indicated at the tree tips, which are colour coded by species. The ancestral node at the centre was defined by midpoint rooting. The outer circle of the heatmap indicates Prostriate or Metastriate lineage. The next two circles of the heatmap indicate the presence of a C-x(3)-C-x(6,10)-C-x(3,6)-C-x(1)-C-x(10,11)-C motif (Cys6-motif) and the numbers of cysteines respectively. The innermost circle of the heatmap indicates the number of CXC-chemokines bound by biochemically characterized evasins. Clades B1 and B2 were defined as having diverged from the two nodes immediately following the ancestral node and are indicated in azure and cyan respectively. Subclades B1.1 and B1.2 were defined as having diverged from the two nodes immediately following the node defining clade B1 and are indicated in light and dark azure respectively.