Effects of GP-17 on the protein expression levels of PI3K/AKT and P38 signaling pathways. (A) Immunoblot analysis of P-PI3K, PI3K, P–P38, P38, P-AKT, AKT, Bcl-2, BAX, BAD, caspase-9, caspase-3, and GAPDH were performed in Langendorff rat hearts; (B and C) bar graphs of the proteins’ relative expression; (D) immunoblot analysis of P-PI3K, PI3K, P–P38, P38, P-AKT, AKT, Bcl-2, BAX, and caspase-9 were performed in H9c2 cardiomyocytes; (E) bar graphs of the proteins relative expression. #P < 0.05 versus the control, ###P < 0.001 versus the control, *P < 0.05 versus the I/R group, **P < 0.01 versus the I/R group, ***P < 0.001 versus the I/R group.GP-17, gypenoside XVII; I/R, ischemia and reperfusion.