Table 1.
Distribution of mutations in the 11 protein sequences of the SARS-CoV-2 genome considering 6 major countries- USA, India, UK, France, Brazil, and Italy relative to the reference strain from Wuhan, China
| Sl no. | Protein sequence of SARS-CoV-2 genome | Length of the protein (amino acids) | Total no. of missense mutations | Percent mutation coverage/mutation density | No. of neutral mutations | No. of deleterious mutations | List of deleterious mutations |
|---|---|---|---|---|---|---|---|
| 1 | ORF1ab | 7096 | 60 | 0.817 | 55 | 5 | L3352F, S3884L, L5030F, H5614Y, V6237F |
| 2 | Spike/ Surface Glycoprotein | 1273 | 24 | 1.88 | 24 | – | – |
| 3 | ORF3a | 275 | 6 | 2.18 | 1 | 5 | P42L, Q57H, G172V, T223I, S253P |
| 4 | Envelope Protein | 75 | 4 | 4.00 | – | 4 | V5A, L37H, P71S, P71L |
| 5 | Membrane Protein | 222 | 0 | – | – | – | – |
| 6 | ORF6 | 61 | 3 | 4.91 | – | 3 | F2M, I11T, I33T |
| 7 | ORF7a | 121 | 1 | 0.82 | – | 1 | V104F |
| 8 | ORF7b | 43 | 0 | – | – | – | – |
| 9 | ORF8 | 121 | 1 | 0.82 | 1 | – | – |
| 10 | Nucleocapsid Protein | 419 | 9 | 2.14 | 8 | 1 | S194L |
| 11 | ORF10 | 38 | 0 | – | – | – | – |
The account of deleterious mutations was obtained from the PROVEAN algorithm. The mutation density was calculated as a function of the total number of mutations points divided by the length of the sequence corresponding to different protein regions of the SARS-CoV-2 genome