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Design of RT-MIRA-based assay for detection of SARS-CoV-2. (A) Alignment of the amplicon with SARS-CoV-2. (B) Schematic of duplex RT-MIRA and lateral flow dipsticks readout for detection of SARS-CoV-2. C line, Quality Control; T1 line, anti-DIG; T2 line, anti-FAM.
