Skip to main content
. 2021 Oct 22;11:678703. doi: 10.3389/fcimb.2021.678703

Figure 2.

Figure 2

Screening of the primers and probes’ candidates for detection of SARS-CoV-2 by using RT-MIRA. (A) Two percent agarose electrophoresis analysis of seven primer pairs used to amplify the ORF1ab gene at 38°C for 30 min. (B) Two percent agarose electrophoresis analysis of nine primer pairs used to amplify the N gene of SARS-CoV-2 at 38°C for 30 min. (C) LFD readout of amplicons of ORF1ab gene and N gene using probes and primers. SARS-CoV-2 national reference at 5 × 104 copies/ml was used as template.