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. 2021 Oct 22;11:678703. doi: 10.3389/fcimb.2021.678703

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Copyright © 2021 Chen, Sun, Wang, Gao, You, Yu, Sun, Yang and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of four collection buffers on amplification efficacy. (A, B) LFD readout analysis of RT-MIRA amplicons in different collection buffers. SARS-CoV-2 RNA with 5 × 10⁴ copies or 5 × 10¹ copies/ml were used as template. (C) Relative intensity signaling of the bands was calculated by ImageJ software. a, the extracted SARS-CoV-2 RNA in DEPC water; b, SARS-CoV-2 national reference in UTM1; c, SARS-CoV-2 national reference in UTM2; d, SARS-CoV-2 national reference in media with 30% guanidine; e, SARS-CoV-2 national reference in UTM3.