Fig. 5.

Rg1 treatment decreased the level of ROS and the expressions of NADPH oxidase 2 in APP/PS1 mice. (A) The ROS production in the cortex, hippocampus CA1 and CA3 (DHE fluorescence staining, 400 × , the bar = 50 μm); (B-D) The mean density of ROS production in the cortex and hippocampus CA1, CA3. (E) The bands of NOX2, p22phox, p47phox and β-actin (Western blot); (F–H) The relative expression of NOX2, p22phox and p47phox over WT-9M; (I–K) The relative mRNA expression of NOX2, p22phox and p47phox (q-PCR). Results are expressed as mean ± SD, n = 4. ∗P < 0.05, ∗∗P < 0.01 vs WT-9M group; ^#P < 0.05, ^##P < 0.01 vs APP/PS1-6M group; ^ΔP<0.05, ^ΔΔP<0.01 vs APP/PS1-9M group.