Figure 1.

BCAR3 is upregulated in breast cancer samples and TNBC tumor cell lines. (A) Human tissue samples were obtained from the University of Virginia Biorepository and Tissue Research Facility (BTRF) and stained for H&E and BCAR3. Scale bars represent 200 μm. Staining intensity was evaluated by 2-3 investigators on a scale of 0 (no staining) to 3+ (high intensity) (see Panel B below). Data shown are the average of 10 fields per sample for 56 normal breast tissues samples, 28 DCIS samples, and 82 IDC samples. The Kruskal-Wallis test was used to determine differences between groups. ***indicates P<0.0001. (B) Examples of BCAR3 staining intensities from breast tissue microarray samples obtained from the BTRF. Scale bars represent 200 µm. The maximum intensity for BCAR3 staining was assessed by 2-3 investigators in 41 normal samples, 40 TNBC samples and 91 ER+/PR+ samples. Data shown are the percentage of samples exhibiting the indicated maximum staining intensities +/- SEM. (C) Representative immunoblot from 11 TNBC cell lines. Lysates from 40,000 cells were separated by SDS-PAGE and immunoblotted for BCAR3, MET, and ERK 1/2. Samples were derived from the same experiment and processed in parallel on multiple blots. (D) Kaplan-Meier plot showing survival data for 255 TNBC patients separated by the top (red) or remaining (black) quartiles of BCAR3 expression in the primary tumor (https://kmplot.com/analysis/index.php?p=service&start=1) [25].