Figure 3.

Restriction enzyme digestion of DNA into fragments, gel separation of restriction products and detection of variation in Varieties I, II and III. (A). Analysis of a section of DNA (long thick black line) of three Varieties I, II and III using a restriction enzyme and three designed probes (green, red and blue short lines). Location of mutations is shown as red asterisk. (B). Gel electrophoresis of the restriction enzyme digested DNA samples of the three Varieties I, II and III. The restriction digestion procedure generates a huge number of fragments with varying lengths in the reaction solution. Upon electrophoresis, the huge number of DNA fragments show virtually as lane of DNA smear on the gel for each variety. (C). Southern hybridization using three designed probes to detect variation among the three varieties I, II and III.