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. 2021 Oct 22;12:712601. doi: 10.3389/fmicb.2021.712601

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Copyright © 2021 Kornspan, Brendebach, Hofreuter, Mathur, Blum, Fleker, Bardenstein and Al Dahouk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of B. melitensis field isolate (first row) and Rev.1 (third row) spectra against the type strain B. melitensis 16M (second row) and a diversity set of B. melitensis strains (fourth row) for selected masses. (A) Putative biomarkers (vertical dotted lines) described by Christoforidou et al. (2020). All spectra in this study displayed a peak at m/z 3,528. Most Rev.1 strains as well as the B. melitensis type strain 16M displayed a peak at m/z 7,328. (B) All biomarker decision boundaries (vertical dashed lines) in this study demarcate B. melitensis Rev.1 from B. melitensis 16M and field isolates. (C,D) Spectra from a diversity set of B. melitensis MVLA8 genotypes (purple) are plotted against a group spectrum of B. melitensis Rev.1 samples (red). For clarity, only spectra acquired at the BfR are shown.