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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Vascul Pharmacol. 2021 Jun 9;139:106881. doi: 10.1016/j.vph.2021.106881

Figure 6. Inflammasome modulates Ang-II-induced vascular oxidative stress in vivo, but not in vitro.

Figure 6.

Representative dihydroethidium (DHE) staining (in red) of transverse aortic sections (×400) (A) and DHE fluorescence quantification of aortic sections (B) from Ctrl (WT) and Casp1−/− mice treated with Ang-II (490 ng/min/kg for 14 days with ALZET osmotic minipumps). ROS measurement in Rat Aortic Vascular Smooth Muscle Cells (RASMC) by 2′,7′-Dichlorodihydrofluorescein diacetate (DCFDA) incubated with Ang-II (0,1 μM) for 30 or 60 min and 8 h or 24 h. Some experiments were performed in the presence of a selective NLRP3 antagonist (MMC950, 1 μM, 30 min). N = 3–5 in vascular tissues and N=5–8 in RASMC. Values are reported as mean ± s.e.m. *P<0.05 vs. WT; #P<0.05 vs. vehicle; **P<0.05 vs MCC950 alone.