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. 2021 Oct 22;11:768222. doi: 10.3389/fonc.2021.768222

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Copyright © 2021 Tan, Yi, Zhang, Tang, Xu and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The sonography and microscopy observation of the tumor-bearing mouse spleen. (A) The two-dimensional and color Doppler flow images of the tumor-bearing mouse spleen (shown in blue arrows) with a small animal ultrasound system (Vevo2100, USA). (B) The two-dimensional and contrast-enhanced ultrasound sonography on the diagnostic–therapeutic ultrasonic apparatus VINNO 70 (VINNO Technology Co. Ltd, Suzhou, China). The region of interest (ROI) is shown by the orange box. Respective H&E-dyed slice observation of the tumor-bearing mice instantly and 24 h after ultrasound-targeted microbubble destruction (UTMD) treatment in treated (C) and control (D) groups.