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. 2021 Sep 29;297(5):101231. doi: 10.1016/j.jbc.2021.101231

Figure 3.

Figure 3

Dynamics of TDP-43 expression during prophase I of meiosis. Immunofluorescence of meiotic chromosome spreads from WT mice showing the dynamics of TDP-43 expression during prophase I of meiosis. Antibodies to TDP-43 (green) and SYCP3 (red) were used; nuclear counterstaining was done with DAPI (blue). TDP-43 is predominantly expressed between midpachytene to late diplotene stages of prophase I (C–F). Very little TDP-43 expression was found in zygotene, early pachytene, and diakinesis stages (A, B, and G, respectively). Quantification of TDP-43 expression is shown in panel H. Panel I shows a diplotene spermatocyte of WT mouse stained with antibodies to TDP-43, SYCP3, and DAPI. The cKO mice showed no expression of TDP-43 and various types of defects occurring at pachytene-like (K–M) and diplotene-like (J) spermatocytes. Arrowhead in (J) indicates synapsis partner exchange, and the arrow in (M) indicates an extra-long chromosome. The scale bar represents 10 μm. cKO, conditional KO; DAPI, 4′,6-diamidino-2-phenylindole; SYCP3, transactive response DNA-binding protein of 43 kDa; TDP-43, transactive response DNA-binding protein of 43 kDa.