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. 2021 Sep 29;297(5):101231. doi: 10.1016/j.jbc.2021.101231

Figure 4.

Figure 4

Loss of TDP-43 causes meiotic failure in cKO mice.A, percentage of cells at different meiotic stages in TDP-43 WT (orange) and conditional knockdown mutant (blue). Most spermatocytes are at late pachytene or early diplotene stage in WT, but accumulation at midpachynema stage occurred in Tardbp cKO. B–E, Tardbp cKO spermatocytes with extensive γH2AX showing nonhomologous chromosome pairing. B, WT pachytene spermatocytes. γH2AX staining covers the sex body. C–E, γH2AX persists in pachytene-like spermatocytes with synapsis defects from Tardbp cKO. γH2AX staining represents nonhomologous synapsed regions; γH2AX foci/flares on autosomes likely indicate unrepaired DSBs. The scale bar represents 10 μm. cKO, conditional KO; DSB, double-strand break; TDP-43, transactive response DNA-binding protein of 43 kDa.