Fig. 3.
Tube method to evaluate biofilm production, developing with crystal violet. A: positive biofilm production, with biofilm attached to the tubefor Pseudomonas aeruginosa (P16) (A1) and Pseudomonas alcaligenes (P18) (A2). B: negative production of biofilm (no presence of biofilm attached to the tube) for Pseudomonas aeruginosa (P25) .