KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Antibodies | ||
|
| ||
| Brilliant Violet 605 anti-mouse CD4 clone GK1.5 | Biolegend | Cat# 300555; RRID:AB_2564390 |
| eFluor 450 anti-mouse CD4 clone GK1.5 | eBioscience | Cat# 48–0041-82; RRID:AB_10718983 |
| Brilliant Violet 650 anti-mouse CD8b clone H35–17.2 | BD Biosciences | Cat# 740552; RRID:AB_2740253 |
| FITC anti mouse CD8b clone H35–17.2 | eBioscience | Cat# 11–0080-85; RRID:AB_657775 |
| PE/Cyanine7 anti-mouse/human CD11b clone M1/70 | Biolegend | Cat# 100215; RRID:AB_312798 |
| eFluor 450 anti-mouse CD11c clone N418 | eBioscience | Cat# 48–0114-80; RRID:AB_1548665 |
| PE anti-mouse/human CD44 clone IM7 | BD PharMingen | Cat# 553134; RRID:AB_394649 |
| PE-Cy7 anti-mouse/hman CD44 clone IM7 | Biolegend | Cat# 103030; RRID:AB_830787 |
| PerCP/Cyanine5.5 anti-mouse/human CD44 clone IM7 | Biolegend | Cat# 103032; RRID:AB_2076204 |
| Alexa Fluor 488 anti-mouse CD45.1 clone A20 | Biolegend | Cat# 110718; RRID:AB_492862 |
| APC anti-mouse CD45.2 clone 104 | eBioscience | Cat# 17–0454-82; RRID:AB_469400 |
| Alexa Fluor 700 anti-mouse CD62L clone MEL-14 | Biolegend | Cat# 104426; RRID:AB_493719 |
| PE/Cyanine7 anti-mouse CD62L clone MEL-14 | Biolegend | Cat# 104417; RRID:AB_313102 |
| APC anti-mouse IFNγ clone XMG1.2 | eBioscience | Cat# 17–7311-82; RRID:AB_469504 |
| FITC anti-mouse IFNγ clone XMG1.2 | BD PharMingen | Cat# 554411; RRID:AB_395375 |
| PE anti-mouse IL-4 clone 11B11 | Biolegend | Cat# 504104; RRID:AB_315318 |
| PerCP/Cyanine5.5 anti-mouse IL-10 clone JES5–16E3 | Biolegend | Cat# 505028; RRID:AB_2561523 |
| APC anti-mouse IL-17A clone TC11–18H10.1 | Biolegend | Cat# 506916; RRID:AB_536018 |
| PE anti-mouse MHC Class II (IA/IE) clone M5/114.15.2 | eBioscience | Cat# 12–5321-82; RRID:AB_465928 |
| Brilliant Violet 421 anti-mouse T-bet clone 4B10 | Biolegend | Cat# 644815; RRID:AB_10896427 |
| PE-Cy7 anti-mouse T-bet clone 4B10 | eBioscience | Cat# 12–5825-82; RRID:AB_925761 |
| Brilliant Violet510 anti-mouse TCRb clone H57–597 | Biolegend | Cat# 109233; RRID:AB_2562349 |
| CyChrome anti-mouse TCRb clone H57–597 | BD PharMingen | Cat# 553173; RRID:AB_394685 |
| PE anti-mouse TCR-vb8.3 clone 1B3.3 | BD PharMingen | Cat# 553664; RRID:AB_394980 |
| Biotin anti-mouse NK1.1 clone PK136 | eBioscience | Cat# 13–5941-82; RRID:AB_466804 |
| Biotin anti-mouse CD11c clone N418 | eBioscience | Cat# 13–0114-82; RRID:AB_466363 |
| Biotin anti-mouse CD8a clone 53–6.7 | eBioscience | Cat# 13–0081-82; RRID:AB_466346 |
| Biotin anti-mouse MHC class II clone M5/114.15.2 | eBioscience | Cat# 13–5321-82; RRID:AB_466662 |
| Purified anti-mouse CD16/CD32 clone 2.4G2 | BD PharMingen | Cat# 553141; RRID:AB_394656 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| LCMV GP61–80 | AnaSpec | Cat# AS-64851 |
| Collagenase D | Roche | Cat# 11 088 858 011 |
| Propidium Iodide Solution | Sigma-Aldrich | Cat# P4864 |
| Lipopolysaccharides from E. coli | Sigma-Aldrich | Cat# L2880 |
| Medium 199 | GIBCO | Cat# 12350039 |
| RPMI 1640 | GIBCO | Cat# 11875119 |
| Foundation Fetal Bovine Serum | Gemini Bio | Cat# 900–108 |
| HEPES | GIBCO | Cat# 15–630–080 |
| Adenine | Sigma-Aldrich | Cat# A2786 |
| Biotin | Sigma-Aldrich | Cat# B4501 |
| Hemin | Sigma-Aldrich | Cat# 51280 |
| PBS | Quality Biological | Cat# 119–069-101 |
| Ficoll Paque Plus | GE Healthcare | Cat# 17–1440-02 |
| Lectin from Arachis hypogaea (peanut) | Sigma-Aldrich | Cat# L6135 |
| Sodium Pyruvate | GIBCO | Cat# 11360070 |
| Antibiotic-antimycotic | GIBCO | Cat# 15240062 |
| 2-Mercaptoethanol | Sigma-Aldrich | Cat# M6250 |
| Brefeldin A | eBioscience | Cat# 00–4506-51 |
| EDTA | Quality Biological | Cat# 351–027-101 |
| Sodium azide | Sigma-Aldrich | Cat# S2002 |
| Phorbol 12-myristate 13-acetate | Sigma-Aldrich | Cat# P8139 |
| Ionomycin calcium salt | Sigma-Aldrich | Cat# I0634 |
| Triethanolamine | Sigma-Aldrich | Cat# 90278 Critical commercial assays |
|
| ||
| Critical commercial assays | ||
|
| ||
| Mouse IL-17A (homodimer) ELISA Ready-SET-Go! | eBioscience | Cat# 88–7371 RRID:AB_2575104 |
| Mouse IFN gamma ELISA Ready-SET-Go! | eBioscience | Cat# 88–7314 RRID:AB_2575069 |
| Mouse IL-2 Quantikine ELISA Kit | R&D Systems | Cat# M2000 |
| Mouse IL-4 Quantikine ELISA Kit | R&D Systems | Cat# M4000B |
| Mouse IL-12 p40 Quantikine ELISA Kit | R&D Systems | Cat# M1240 |
| Mouse IL-12 p70 Quantikine ELISA Kit | R&D systems | Cat# M1270 |
| Dynabeads M-280 Streptavidin | Invitrogen | Cat# 11205D |
| Cytofix/Cytoperm | BD Biosciences | Cat# 554722 |
| Foxp3 / Transcription Factor Staining Buffer Set | eBioscience | Cat# 00–5523-00 |
| LIVE/DEAD Fixable Near-IR | Invitrogen | Cat# L10119 |
| Ghost Dye Red 780 | Tonbo Biosciences | Cat# 13–0865 |
| BCA Protein Assay Kit | Pierce | Cat# 23225 |
|
Deposited data | ||
| GTEx Analysis Release V8 (dbGaP Accession phs000424.v8.p2) | GTEx | https://www.gtexportal.org/home/gene/IL12B |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| L. major-RFP | Kimblin et al., 2008 | Kimblin et al., 2008 |
| Mouse: B6.129S1-Il12btm1Jm/J | The Jackson Laboratory | Cat# 002693 |
| Mouse: IL-12p40−/− CD45.1+ | Bred in-house | N/A |
| Mouse: C57BL/6J | The Jackson Laboratory | Cat# 000664 |
| Mouse B6.SJL-PtprcaPepcb/BoyJ | The Jackson Laboratory | Cat# 002014 |
| Mouse: B6NTac | Taconic Biosciences | Cat# B6 |
| Mouse: B6.SJL-Ptprca/BoyAiTac | Taconic Biosciences | Cat# 4007 |
| Mouse: B6 1x2 | Bred in-house | N/A |
| Mouse: B6.129S1-Il12atm1Jm/J | The Jackson Laboratory | Cat# 002692 |
| Mouse: B6.Cg-Ptprca Pepcb Tg(TcrLCMV)1Aox/PpmJ | The Jackson Laboratory | Cat# 030450 |
|
Software and algorithms | ||
| FlowJo (v10.6.2) | BD Biosciences | https://www.flowjo.com/solutions/flowjo/downloads |
| Prism 7.05 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| GTEx Portal | Broad Institute of MIT and Harvard | http://www.gtexportal.org/home/index.html |