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. 2021 Oct 22;12:761910. doi: 10.3389/fphys.2021.761910

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Copyright © 2021 Gao, Li, Liang, Yu, Liu, Ni and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression and localization of KCNQ1 and KCNE1 in human sperm. (A) Human sperm were lysed after capacitation for 3h. Sperm proteins were separated and analyzed using 10% SDS-PAGE and Western blotting, using antibodies for KCNQ1 (Abcam) and KCNE1 (Abcam). The figure is representative of 3 separate experiments. The full uncropped immunoblots was provided in the supplementary data (Supplementary Figure 1). (B) Indirect immunofluorescence of KCNQ1 (Santa Cruz) and KCNE1 (Invitrogen) in human sperm before and after capacitation. KCNQ1 (red), KCNE1 (green), and co-localization of KCNQ1 and KCNE1 (merge). The negative control cells were incubated with normal IgG as the primary antibodies. The nuclei of spermatozoa were stained blue with DAPI. The figure is representative of 3 separate experiments.