Skip to main content
. 2021 Oct 22;12:761910. doi: 10.3389/fphys.2021.761910

Figure 2.

Figure 2

Chromanol 293B effect on human sperm AR and viability. (A) The human sperm AR was evaluated using PSA-FITC staining. Sperm were treated with different reagents (DMSO or 100μM chromanol 293B) for 3h during capacitation, and the sperm were then treated with 15μM progesterone for 15min to induce AR. To detect spontaneous AR before capacitation, sperm were stained with PSA-FITC immediately after discontinuous Percoll gradient centrifugation and washing. AR indicates acrosome-reacted sperm, while acrosome integrity (AI) indicates uncapacitated sperm. (B) After PSA-FITC staining, sperm were examined by fluorescence microscopy. The AR ratio is calculated by dividing the number of AR sperm with the total number of sperm. For 100 μM chromanol 293B group compared to vehicle control group, *p<0.05. Propidium iodide (PI) is used to stain dead sperm. Percentage of not viable cells (NVC%) was calculated. Values represent the means ± SEM of at least 6 experiments.